FOR ORE INFORMATION Europe and other ountries 33(0)466 96 0 U. Incubate overnight at RT. Plate 25 μL of cells in. (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based). FOR HTRF CERTIFIED READER. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. 96-well culture plateHTRF certified . Both Microplate Optimization and Read Height Adjustment were. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations. 47 seconds for a 1536-well plate. In particular, these parameters should be entered as below. Cover the plate with a plate sealer. Plates can be loaded manually or plates can be loaded using 20 or 50-plate free access magazines which fit in a stacker. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. * For HTRF microplate recommendations, please visit . 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same . MATERIALS PROVIDED: KIT . Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. FOR HTRF CERTIFIED READER For. Read & analyse. Envision Nexus. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The Z´ value takes into account four (4) parameters: the means (µ) and the standard deviations (σ) of the positive (plateau) and the negative (baseline) controls 1. Remove the plate sealer and read on an HTRF® compatible reader . It fits any benchtop – and budget Building on our pioneering family of benchtop multimode plate readers, the VICTOR® Nivo™ multimode reader breaks new ground, packing all popular detection modes into the industry’s smallest benchtop. For more information about HTRF ®• HTRF®-Certified Reader. SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. Make sure the setup for Tb Cryptate is used. Make sure the setup for Tb3+ Cryptate is used. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. FOR HTRF CERTIFIED READER. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. The ratio between control lysate signal / non-specific signal should be greater than 2. For more information about HTRF. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Plate Type and Read Area should be selected based on the microplate used and wells to be read. Its. HTRF Signal + 2 hours @ RT [Cortisol] ng/ml 10 µL Standard or Sample 5 µL Cortisol acceptor reagent 5 µL Cortisol donor antibody ADD READ & ANALYSESafire 2™ readers must be appropriately be configured for HTRF readout by setting up the measurement conditions in the “multilabeling” function of Magellan software. 1. For more information about HTRF. FOR HTRF CERTIFIED READER. HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. Lysates are ®then transferred to the assay plate for the detection of phospho- Rab10 (Thr73) by HTRF reagents. COMPONENTS ; STORAGE . An. Microplate Readers and Reader Accessories. Plate 25 μL of cells in. Lysates are then transferred to the assay plate for the detection of total-Rb by HTRF. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. We would like to show you a description here but the site won’t allow us. Setu CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Its. 4. Microplate readers are only certified if they. Data Analysis HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. 10 μL of GLP1 receptor cells were dispensed into wells of. PLATE READER INTRODUCTION Selecting the right plate reader for your lab can be challenging. No washing steps are required. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates to a low volume detection plate (either HTRF 384-lv or 96-lv plate) before the addition of HTRF Phospho-Rb (Ser780) detection reagents. For more. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Your lab might even have one already. best fluorescence polarization plate reader on the market. . pre+ 5 µL HTRFcompounds Activation Buffer. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Partnerships for delivering turn-key solutions. 8 µL cells. There are multiple factors to consider including: detection type, additional. Remove the plate seal and read on an HTRF compatible reader. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. 55 µL anti-cAMP-Cryptate. Plate cells Stimulate cells 3. The samples were each placed in a well of a 24-well plate, covered with 1 ml of culture medium, and incubated for a total of 17 days. FOR HTRF CERTIFIED READER. 4 mg/ml. Read & analyse. For more ® information about HTRF compatible readers and for set-up recommendations. HTRF Signal + 16 µL 4 µL Standard of pre-mixed IL8 Antibodies or Sample ADD READ ANALYSE Incubate 1 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. PROTOCOL AT A GLANCE Do not pre-mix the d2 and Cryptate solutions prior to dispensing. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. 5 EXAMPLE OF MAP PLATE . Each well requires 10 μL of 4X lysis buffer /well. Plate 25 μL of cells in. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. FOR HTRF CERTIFIED READER. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Reagents were dispensed following Cisbio’s ligand binding protocol in 384-well format (white plates). SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4 SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. For more information about HTRF ®Seal the plate and incubate for Overnight. Lysates are then transferred to the assay plate for the detection of total-CREB by HTRF. 5% and an increased Z’ value of 0. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Introduction. Discover the features and benefits of the VICTOR Nivo multimode plate reader, the smallest and most versatile instrument for life science applications. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. • HTRF®-Certified Reader **. NON-SPECIFIC BINDING TOTAL BINDINGCover the plate with a plate sealer. Designed to the highest standards to support your drug discovery research, Corning ® and Falcon ® microplates are ideal for drug discovery applications, including assay prep, assay development, and HTS and automation. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. These plates can be read on all HTRF certified readers, thus making their implementation in. The Bradford assay was successfully performed on the BMG LABTECH microplate reader. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. FOR HTRF CERTIFIED READER. Homogeneous Time-Resolved Fluorescence (HTRF) . acceptor reagent. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. Small ONE- PLATE ASSAY PROTOCOL: overnight FOR HTRF CERTIFIED READER . 25,000 human Jurkat cells were plated in 96 well plates. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. EX: HTRF Emission range. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. These plates can be read on all HTRF certified readers, thus making their implementation in. 3nm, important when reading at 280 nm for DNA analysis, for more exact results. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. It also integrates with the powerful SoftMax Pro Software for. Filters or monochromators for emission wavelength. For more information. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. Read & analyse. . The EnSight<sup>®</sup> multimode plate reader offers HTS Alpha and Ultra-sensitive Luminescence technologies alongside conventional multimode detection technologies. Excitation lasers significantly improve performance and lower. Revvity. FOR HTRF CERTIFIED. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance forCisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. The signals are produced by, converted by, or transmitted through the. Incubate overnight at RT. The PHERAstar ® FSX and CLARIOstar ® microplate readers offer the best possible performance for AlphaScreen ®, AlphaLISA ®, and AlphaPlex™ assays. The system provides even higher sensitivity for time-resolved fluorescence (TRF) applications. Lysates are then transferred to the assay plate for the detection of total-p53 by HTRF. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. • User-upgradable microplate reader in under 2 minutes • Array of detection cartridge applications at your fingertips. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. 9. Lysates are then transferred to the assay plate for the detection of Total Cofilin by HTRF® reagents. + 4 µL HTRF pre-mixed antibodies. The adapterResults summary for HTRF IP1 standard curve. FOR HTRF CERTIFIED READER. Because of its homogeneous and fast nature, the assay is a preferred method to determine the protein concentration of samples. Step 5 Remove the plate sealer and read on an HTRF® compatible reader The blank control is used to check the Cryptate signal at 620 nm. If not, our scientific team will help you choose the instrument that best suits your needs and budget. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. For more ® information about HTRF compatible readers and for set-up recommendations, please. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 5 hours before 3 more washing steps. 3 and higher include optimized reader settings, as well as data analysis, for faster results. It is used as a positive control for Gs applications ( biological models validation,Detection of Total CDK12 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. No washing steps are required. Handle complex biological matrices easily. FOR HTRF CERTIFIED READER culture plate to a 384-well small volume white plate. Read & analyse. FOR HTRF CERTIFIED READER. NC1286225. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The HTRF ratio obtained represents the total binding. For more information about HTRF. Figure 3. An example of a LanthaScreen kinase assay run on a BMG LABTECH plate reader can be seen in the application note " LanthaScreen TR-FRET tyrosine kinase and protein kinase C assay ". Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. This is thanks to a dedicated Alpha Technology laser which outperforms any xenon-based light source by exciting donors at 680 nm. It continues to deliver optimal results. Flexibility combined with the best sensitivity of its class, and our newly developed Enhanced Dynamic Range technology, make it the ideal reader for assay development. Revvity. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Plate 25 μL of cells in. 96-well culture plateFOR HTRF CERTIFIED READER. But sometimes these parameters are not sufficient enough. a one- plate assay protocol. and TR-FRET, for example to detect PerkinElmer’s DELFIA, HTRF and LANCE assay technologies Time resolved fluorescence certified for use with HTRF. For more information about HTRF. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 1) . Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Reader. 5 μL of Binding buffer 2X. The Dual-Luciferase ® Reporter Assay or DLR is widely used to study gene transcription and regulation. Thanks to HTRF signal stability, the assay plate can be used to check several instruments, or different reader. Seal the plate and incubate 1 hour at RT : Remove the plate sealer and read on an HTRF® compatible reader . For more information. HTRF-specific. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERHTRF Ratio Anti-Tag Antibody Cryptate Inhibitor-Red Inhibitor Purified Tagged Kinase Figure 2: Principle of HTRF kinase competition binding assay (IC 50 - KI determination) HTRF Dasatinib-Red Part # 62KB02REDC & 62KB02REDE Amount: 1 nmol (62KB02REDC) & 20 nmol (62KB02REDE) Concentration: 25 µM in DMSO Form:. FOR HTRF CERTIFIED READER. After an overnight incubation, the plate is washed 3 times. After defining the objectives and their locations in Gen5: 1. Incubate 4h at room temperature. At that stage, the analyte is sandwiched between the 2 antibodies. The CLARIOstar® Plus is our most flexible multi-mode plate reader. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Configure your microplate reader. 5. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Plate 25 μL of cells in. In addition, stacker. HTRF is compatible with a large range of available microplate readers. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Make sure the setup for Eu 3+ Cryptate is used. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYFOR ®READING, AN HTRF -CERTIFIED READER IS NEEDED. Plate 25 μL of cells in. For more information. 45mm 5000µs Delay time Reading time Operation mode 100 µs 400 µs by plate 100 µs 400 µs by plate • This reader only allows high performance HTRF measurement when assays are run in WHITE plates. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. FOR HTRF CERTIFIED READER For more information about HTRF. It can also be further streamlinedFOR HTRF CERTIFIED READER. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Table 1. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. Read other about this measurement approach here. The ratio of the two fluorescence Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. If not, our scientific team will help you choose the instrument that best suits your needs and budget. FOR HTRF CERTIFIED READER For more information about HTRF. Specialized filters allow for IR fluorescence. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. + 4 µL HTRF ® pre-mixed antibo dies 2. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERDetection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. View Brochure. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. The EnVision XCite Plate Reader provides exceptional sensitivity and speed with minimal downtime - backed by our global network of application scientists and engineers. Make sure the setup for Tb Cryptate is used. Concentrate on running your assays instead of running your instrument: the optic modules contain all the filters, dichroics, and more needed to run your assay. Catalog No. It features a modular design that allows users to add or change detection cartridges according to their needs. STANDARD PROTOCOL FOR INHIBITORY ASSAY IN 20 μL FINAL VOLUME Standard Inhibitor Tag1-TNFα Tag2-TNFR1 Anti-Tag1 Eu Cryptate antibody Anti-Tag2 XL665 reagent PPI Europium detection bufferThe perfect microplate reader for everyday biochemical and cell-based assays. Your lab might even have one already. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. S. DLReady™ certified luminometer plate readers. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED. White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. The detection antibody is incubated for 1. Using a black. HTRF combines time-resolved fresh detection with FRET and is mainly used for drug screening in high-throughput. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. These are the different kinetic modes on BMG LABTECH microplate readers. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. This protocol, HTS designed, allows miniaturization while. at room temperature STEP 4 Remove the plate sealer and read on an HTRF ® compatible reader. Plus . For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed IL1β Antibodies ADD READ ANALYSE Incubate overnight* at room temperature *Reading possibe after 2h White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed TNFα Antibodies ADD READ ANALYSE Incubate 2 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. Application Area: absorbance, fluorescence, luminescence, and homogeneous time resolved fluorescence assay development. Lysates are then transferred to the assay plate for the detection of phospho-Tie2 (Tyr992) by HTRF. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. The ratio for each well must be calculated individually. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. It can also be further streamlined to TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large range of available microplate readers and certainly the detection system already in your lab. For more information about HTRF. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. For a list of HTRF-compatible readers and set-up recommendations, please visit . It is used as a positive control for Gi applications ( biological models validation,FOR HTRF CERTIFIED READER. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. EnSight ® offers both fast well-imaging and multimode detection capabilities for target-based and phenotypic screening, all in a single benchtop instrument. 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The PHERAstar ® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. ® Detection of Progesterone Receptor with HTRF reagents is performed in a single plate used for plating, stimulation and detection. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Plate 25 μL of cells in. Plus . ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. FOR HTRF CERTIFIED READER. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detectionThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Plate 1 plate HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. Its unique optical design and instant polarizer switching provide the smallest mP standard deviation in any assay. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. The assay is then ready to be read on an HTRF compatible or certified microplate reader. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. It utilises an ultra-fast UV/vis spectrometer or filters for absorbance as well as highly sensitive filters for all other detection modes. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. First the plate is coated with a capture antibody. The donor and acceptor can be used to label a variety of biomolecules, for. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. For more information about HTRF. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For more information. Read & analyse. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for• ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. culture plate. It can also be further streamlined toFOR HTRF CERTIFIED READER. 1. Experimental details• HTRF®-Certified Reader **. " The PHERAstar FSX instrument is a high quality, reliable, plate reader. Multimode plate readers include, at the very least, some combination of absorbance (typically covering the UV/visible range), fluorescence and luminescence. Add. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. It measures at any wavelength, with top and bottom reading for most standard technologies. On the SpectraMax i3x reader, install the HTRF cartridge, and wavelengths will be selected automatically when HTRF is selected as the Optical Configuration. Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. If not, our scientific team will help you choose the instrument that best suits your needs and budget. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. 41 mg/ml) was obtained from Thermo Fisher Scientific. It guarantees high level of cAMP accumulation in the cells. Read & analyse. HTRF KinEASE-TK kit and HTRF 96-well low volume plate were obtained from Cisbio Bioassays. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. ** The Detection Buffer is used to prepare working solutions of acceptor and donor reagents. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. For a list of HTRF-compatible readers and set-up recommendations, please visit . Incubate overnight at RT. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. When equipped with a quad monochromator, the EnSight plate reader can improve performance for absorbance and fluorescence detection. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. e. You can verify your reader’s capacity to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Dual-Luciferase® (DLR™) gene assays. FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-3 (Ser473) by HTRF. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. For more information. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. Plate 25 μL of cells in. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Download Setup. Nephelometers are dedicated instruments that can only detect scattering and are only available as standalone plate readers. FLUOstar Omega Filter-based multi-mode microplate reader . support such assays and even feature endorsements from reagent suppliers saying the reader is “HTRF compatible,” “LanthaScreen Certified Plus,” Transcreener Validated” or. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. It accepts all types of microtitration plate. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). 27 / Pack of 100; Qty. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit.